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KMID : 0359320000400020339
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Korean Journal of Veterinary Research
2000 Volume.40 No. 2 p.339 ~ p.343
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Isolation and prevalence of Setaria microfilariae in cattle
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Abstract
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Isolation and identification, prevalence and density of Setaria microfilariae(mf) were investigated in the blood of 406 cattle slaughtered at the abatoirs in Yungnam province from February 1999 to March 2000. Nine ㎖ of blood from the jugular vein was collected in a test tube including EDTA respectively. The blood was mixed(10 : 1) with 0.2% Saponin in saline solution. This mixture was diluted again(1 : 10) with saline solution and stirred. This diluted mixture was filtered using Millipore membrane filter with a pore size of 3.0㎛. Mf were recovered from the membrane in saline solution. Mf isolated were identified as Setaria digitata on the basis of morphologic characteristics.
Infection rate of the mf of total 406 cattle was revealed as 6.40%. Seasonal prevalences of the mf were presented 9.46%(7/74) in Autumn, 7.05%(11/156) in Summer, 4.65%(4/86) in Winter and 4.44%(4/90) in Spring respectively. Average density of the mf of every 9㎖ blood in 26 positive cattle was revealed as 170(2-641) worms. Seasonal densities of the mf of 9㎖ blood were presented average 361(160-641) worms in Autumn, 205(16-620) worms in Summer, 108(6-204) worms in Spring, 6(2-11) worms in Winter respectively.
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